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1.
轮廓与特征点研究是头足类角质颚形态特征鉴别的基本方法,对于轮廓与特征点的提取最常用的方法是手动描绘与标定,利用计算机视觉进行轮廓与特征点的提取,不仅可以降低手动提取带来的误差、提高准确性,而且更加快速、便捷。文章将利用计算机视觉提取头足类角质颚的轮廓与特征点,首先将自制装置拍摄得到的角质颚三视图放入MTALAB软件中进行编程处理,然后利用Canny算法提取角质颚轮廓,最后根据地标点的定义标定特征点位置并建立空间坐标系得到角质颚的特征点坐标。研究结果显示,利用计算机视觉提取角质颚的轮廓图像以及特征点坐标是可行的,当标准差σ取值为0.1时角质颚轮廓图效果最佳,在得到的轮廓图上进行特征点的标定,通过迭代遍历轮廓图获得各个特征点的空间坐标。研究分析认为,将计算机视觉应用于头足类角质颚形态学的研究可以提高研究的便捷性,同时也为后续的研究提供了新的实验思路和方法。  相似文献   
2.
Zhao  Zhonghua  Lin  Jianwei  Fu  Jun  Jiang  Yuwu 《Ocean Dynamics》2020,70(12):1571-1585
Ocean Dynamics - Cold water anomalies were found in the middle layer in the northern Taiwan Strait in the spring of 2015. The cold water was located at a depth of approximately...  相似文献   
3.
为研究南美洲鳗鲡(Anguillarostrata)的耗氧率及其对水温、低溶氧、非离子氨和亚硝酸盐耐受性,本研究采用自制的鱼类呼吸装置测定其耗氧率和窒息点,以黑仔鳗为试验材料,探索其对水温、非离子氨、亚硝酸盐耐受性。结果表明,南美洲鳗鲡耗氧率存在昼夜变化,其昼间耗氧率为(86.46±37.77)mg/(kgh),夜间耗氧率为(123.58±22.56)mg/(kgh),二者存在显著性差异(P0.05);在15—30°C范围内,耗氧率随温度升高逐渐增大,耗氧率和水温的回归方程y=–0.1316x2+9.4507x–13.712(R2=0.9993);南美洲鳗鲡的耗氧率随体质量增大而降低,耗氧量随体质量增加而增大,耗氧量和体重的回归方程为y=0.2321x0.8334(R2=0.9979);在水温25°C时其窒息点随鱼体质量的增大而降低,均重10g、40g和160g的鳗鲡窒息点溶解氧浓度分别为(0.98±0.25)、(0.46±0.06)和(0.32±0.02)mg/L;13—29°C为南美洲鳗鲡的适温范围,25—29°C为其生长适宜水温,在一定范围内的短时低水温或高水温环境对其损伤是可逆的;水中非离子氨对南美洲鳗鲡的LC50和SC分别为12.22mg/L和1.22mg/L,亚硝酸盐氮对南美洲鳗鲡的LC50和SC分别为61.68mg/L和6.17mg/L。  相似文献   
4.
从杂色鲍(Haliotis disversicolor)的表达序列标签(EST)中首先鉴定出β-1,3-葡聚糖识别蛋白基因片段(beta-1,3-glucan recognition protein,saflgrp)。通过5’RACE获得5’端序列,并用由头至趾(headtotoe)PCR方法检测全长cDNA序列的正确...  相似文献   
5.
以大黄鱼(Larimichthys crocea)为材料,对荧光标记微卫星(fSSR)分析体系进行了优化,建立了适合大黄鱼fSSR分析的最佳条件.与常规的银染法相比,该方法成本略高,但其分辨率高,可重复性强,检测效率为银染法的6~10倍.利用优化的fSSR 技术进行了大黄鱼亲于鉴定的研究,结果显示在使用6对引物的情况下...  相似文献   
6.
We estimated the reproductive success of adult brood fish and the heritability of growth-related traits for large yellow croaker (Larimichthys crocea). We created two independent full-factorial cross groups (1 and 2) by crossing 4 males×4 females and 4 males×3 females, respectively. We measured the body weight (BW), body length (BL), and body height (BH) of 281 individuals from group 1 and 318 individuals from group 2 at 20 months post hatch (harvest age). We also collected a tissue sample from each individual. The parents and offspring were genotyped using six polymorphic microsatellites. Of the 599 offspring, 99.2% were assigned to a single pair of parents. In both groups, some parent pairs produced a large number of offsprings while other pairs did not produce any offspring. The genetic diversity and putative Ne were lower in the offsprings than in the parents in both groups. The heritability estimates at 20-month age were 0.13±0.10 for BW, 0.19±0.13 for BL, and 0.09±0.06 for BH. The genetic and phenotype correlation between BW, BL, and BH was close to 1. The results provide basic information for selective breeding and further genetic characterization of large yellow croaker.  相似文献   
7.
于2013-2014 年在三沙湾选择增殖放流区Ⅰ区和Ⅱ区, 开展了2 批次的缢蛏增殖放流, 计 放流缢蛏苗数量约3747 万粒。采用对缢蛏生长、生物量动态变化和滩涂底质主要因子跟踪监测的方 法, 开展对缢蛏增殖放流效果的评估。研究结果, 2 个增殖放流区的缢蛏密度和缢蛏生物量都比对照 区有极显著提高, 共收获65 吨缢蛏大规格苗, 增殖放流产出比为1: 8.0-9.0, 获得了一定的资源增 殖效果和经济效益。放流期间, 滩涂底泥中的氧化还原电位、硫化物和有机碳等浓度在增殖区Ⅰ区、 Ⅱ区和对照区之间都无显著差异(P>0.05), 但, 底泥中Ⅰ区、Ⅱ区的氧化还原电位平均值分别比对照 区提高了26%和18%, 硫化物浓度平均值分别比对照区降低了45%和34%, 有机碳浓度平均值分别 比对照区下降了5.8%和6.8%, 表明, 缢蛏增殖放流获得了一定的改良底质环境效果。  相似文献   
8.
采用池塘中设置生物膜净水栅对比实验的方法,在8口土池开展生物膜"细菌-藻类"协同系统改良池塘养殖水质与沉积物的效果、对苗种培育效果的影响及其相关机理等的研究。结果表明,在142d的养殖期间,处理组比对照组节水减排达65.1%,具有极显著差异(P0.01);处理组水质的p H、总氮和亚硝酸盐氮分别极显著低于对照组3.7%、31.3%和38.7%(P0.01),氨氮、总磷、活性磷和硫化物分别显著低于对照组25.6%、41.6%、37.8%和27.9%(P0.05);弧菌数和藻类密度极显著低于对照组63.1%和51.3%,硅藻相对密度极显著高于对照组93.7%;细菌总数显著高于对照组50.8%,蓝藻相对密度显著低于对照组16.6%;处理组的藻类生物均匀度指数显著高于对照组8.9%;生物膜上的细菌总数高达1.30×109 CFU/g,而弧菌数为零;处理组池塘沉积物的氧化还原电位显著高于对照组30.5%,硫化物浓度显著低于对照组47.0%;草鱼苗种起捕规格、成活率、产量和生长速度分别显著高于对照组24.6%、4.3%、29.8%和28.6%,饲料系数极显著低于对照组15.6%,处理组每公顷池塘培育草鱼大规格鱼种可增加净利润约1.92万元。  相似文献   
9.
P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.  相似文献   
10.
Due to its unique geological location, the Bering Sea is an ideal place to investigate the water exchange and ecosystem connectivity of the Pacific Ocean–Arctic Ocean and subarctic–Arctic region. Based on a number of summer surveys(July to September, 2010, 2012 and 2014), macrobenthic communities and their spatial-temporal patterns are exhibited for the majority of the Bering Sea(53°59′–64°36′N). The results show that the macrobenthic communities were dominated by northern cold-water species and immigrant eurythermic species, and the communities assumed a dispersed and patchy distribution pattern. Polychaetes(Scoloplos armiger), crustaceans(Ceradocus capensis) and sea urchins(Echinarachnius parma) were the main dominant groups in the shallow shelves; the sea star(Ctenodiscus crispatus) and the brittle star(Ophiura sarsii) were the main dominant groups in the continental slope; whereas small polychaetes(Prionospio malmgreni) dominated the basin area. Sediment type, water depth, and currents were the major factors affecting the structure and spatial distribution of the macrobenthic communities. Compared with other seas, the shallow areas of the Bering Sea showed an extremely high-standing biomass. In particular, the northern shelf area(north of St. Lawrence Islands and west of 170°W),which is primarily controlled by Anadyr Water, is an undersea oasis. In contrast, a deficiency in the downward transport of particulate organic carbon has resulted in a desert-like seabed in the basin area. By comparing our results to previous studies, we found that macrobenthic communities of the Bering Sea have undergone significant structural changes in recent decades, resulting in a decrease in abundance and an increase in biomass.In addition, populations of amphipods and bivalves in the northern shelves have decreased significantly and have been gradually replaced by other species. These changes might be associated with advanced seasonal ice melting,changes in organic carbon input, and global warming, indicating that large-scale ecosystem changes have been occurring in the Bering Sea.  相似文献   
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